2023, Issue 7, Volume 15

IN VITRO EVALUATION OF PLANT EXTRACTS, BIO AGENTS AND FUNGICIDES AGAINST ALTERNARIA ALTERNATA INCITANT OF BROWN LEAF SPOT OF POTATO

Dewprakash Patel*, N. Lakpale, A.S. Kotasthaneand Deepti Jha

Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur (Chhattisgarh)

Received-02.07.2023, Revised-14.07.2023, Accepted-25.07.2023

Abstract: Brown leaf spot disease of potato caused by Alternaria alternata has been known as one of the destructive and common dis­eases of potato and occurred worldwide. In vitro evaluation of plant extracts, Euphorbia tithymaloides (Nagdon) was found most effective followed by Jasminum spp (Jasmine) and Eclipta prostrate (Bhringraj). Highest mycelial growth inhibition (71.86%) was recorded at 10% concentration of Trichoderma viride culture filtrate followed by Trichoderma viride (60.74% reduction) and Pseudomonas fluorescens (34.81% growth reduction) over to control. Among the different fungicides, Hexaconazole 4% + Zineb 68% WP at 0.3% concentration exhibited nearly 100% reduction in mycelial growth over to control treatment (90.00 mm radial growth) at par with Hexaconazole 5% EC at 0.2%, Captan 70% + Hexaconazole 5% WP at 0.3% followed by Fluopyram 17.7% + Tebuconazole 17.7% SC at 0.2% with 92.59% mycelial growth reduction and Tebuconazole 50% + Trifloxystrobin 25% SC at 0.2% with 91.48% reduction over to control at 7 DAI.

Keywords: Potato, Alternaria alternata, plant extract, bio agents, fungicide, in vitro

References

Sahar, A. A., Malik, Al-Saadi., Sabeh, D. Alutbi and Zainab, J. Madhi (2017). The effects of in vitro culture on the leaf anatomy of potato (Solanum tuberosum L. CV. Arizaona). International journal of Current Research, 9(7): 54337-54342.

Google Scholar

Amadioha A.C. (2000). Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica. Crop Protection, 19: 287–290.

Google Scholar

Chaitali, V. (2014). Studies on Alternaria cassiae causing leaf blight of cowpea. M.Sc. Thesis, Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur.

Google Scholar

Droby, S., Prusky, D., Dinoor, A. and Barkai- Golan, R. (1984). Alternaria alternata: A new pathogen on stored potatoes. Plant Diseases, 68: 160-161.

Google Scholar

Giha, O. H. (1973). Distribution and morphology of Alternaria tenius associated with leaf spot in Sudan. Transactions of the British Mycological Society, 6: 265-275.

Google Scholar

Gudmestad, N. C. and Pasche, J. S. (2007). Role of fenamidone in the management of potato early blight – Alternaria solani. Special Report no.12. Proc. 10th Workshop of an European network for development of an integrated control strategy of potato late blight. Italy, Applied Plant Research Wageningen UR, 370: 175-182.

Google Scholar

Jansky, S. H., Simon, R. and Spooner, D. M. (2008). A test of taxonomic predictivity: resistance to early blight in wild relatives of cultivated potato. Phytopathology, 98(6): 680–687.

Google Scholar

Kagale S., Marimuthu T., Thayumanavan B., Nandakumar R. and Samiyappan, R. (2004). Antimicrobial activity and induction of systemic resistance in rice by leaf extract of Datura metel against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiological and Molecular Plant Pathology, 65: 91–100.

Google Scholar

Kirk, W. and Wharton, P. (2008). Michigan Potato Disease: Brown Leaf Spot, MSU Extension Bulletin; E3182.  

Google Scholar

Nashwa, S. M. A. and Abo-Elyousr, K. A. M. (2012). Evaluation of various plant extracts against the early blight disease of tomato plants under greenhouse and field conditions. Plant Protect. Sci., 48:74–79.

Google Scholar

Nolte, P. (2008). Brown spot and black pit of potato: the other early blight. American Vegetable Grower, 56 (5):32-33.

Google Scholar

Panse, V.G. and Sukhatme, P.V. (1985). Statistical methods for agricultural workers. Indian Council of AgriculturalResearch Publication, 87-89.

Google Scholar

Patni, C. S., Kolte, S. J. and Awasthi, R. P. (2005). Efficacy of botanicals against Alternaria blight (Alternaria brassicae) of mustard. Indian Phytopathol., 58(4):426-430.

Google Scholar

Rani, S., Singh, R. and Gupta, S. (2017). Development of integrated disease management module for early blight of tomato in Jammu. Journal of Pharmacognosy and Phytochemistry, 6 (2):268-273.

Google Scholar

Roopa, R. S., Yadahalli, K. B. and Kavyashree, K. B. (2014). Evaluation of natural plant extracts, antagonists and fungicides against early blight caused by A. solani in vitro. The Bioscan, 9(3):1309-1312.

Google Scholar

Sharma, R. L., Ahir, R. R., Sharma, A., Ghasolia, R. P., Kumar, N. and Sharma, P. (2022). Management of Alternaria blight (Alternaria alternata) of tomato through novel combined formulations of fungicides. DOI: https://doi.org/10.21203/rs.3.rs-2079637/v1.

Google Scholar

Singh, P.C. and Singh, D. (2006). In vitro evaluation of fungicides against Alternaria alternata. Ann. Pl. Protec. Sci.,14 (2):500-502.

Google Scholar

Soleimani, M. J. and Kirk, W. (2012). Enhance resistance to Alternaria alternata causing potato brown leaf spot disease by using some plant defense inducers. J. Pl. Prot., 52:83-90.

Google Scholar

Thaware, D. S., Fugro, P. A., Jadhav, Y. T., Magar, S. V. and Karande, R. A. (2010). In vitro Evaluation of different fungicide, plants extracts and bio-agents against Alternaria alternata (Fr.) keissler causing leaf blight of cowpea. International J Pl. Protection, 3(2):356-360.

Google Scholar

Tsedaley, B. (2014). Review on early blight (Alternaria spp) on potato disease and its management options. J Biol Agric Healthc., 4:191-198.

Google Scholar

Van der Waals, J. E.,  Pitsi, B. E., Marais, C. and Wairuri, C. K. (2011).  First report of Alternaria alternata causing leaf blight of potatoes in South Africa. Plant Dis., 95(3):363.

Google Scholar

Varma, J. and Dubey, N. K. (1999). Prospectives of botanical and microbial products as pesticides of tomorrow. Current Science, 76:172-179.

Google Scholar

Zade, S. B., Ingle, Y. V. and Ingle, R. W. (2018). Evaluation of fungicides, botanicals and bio-agents against Alternaria alternata incitant of leaf spot of soybean. Journal of Pharmacognosy and Phytochemistry,7(5):1687-1690.

Google Scholar