2023, Issue 3, Volume 15

STABILIZATION OF BIOLOGICAL EFFICIENCY OF ISOLATES OF VOLVARIELLA VOLVACEA (BULL EX. FR.) SINGER THROUGH SINGLE SPORE CULTURES

Sharad Shroff1* and Chandrakanta Soni2

¹ Indira Gandhi KrishiVishwavidyalaya Raipur Chhattisgarh

² AtalBihari Vajpayee VishwavidyalayaBilaspur, Chhattisgarh

Received-02.03.2023, Revised-11.03.2023, Accepted-26.03.2023

Abstract: An experiment was conducted to stabilize the variable biological efficiency of Volvariella volvacea through single spore culture. Pure culture of Volvariella volvacea raised from tissue culture, multispore culture and monospore culture are studied for the radial growth, Biomass & Chlamydospore production, also studied for growth and yield parameters significantly highest yield (1.530 kg) & biological efficiency (21.86%) in single spore culture (SSI 3) found statistically at par  with tissue culture of BYT VV-05 isolate with yield (1.410 kg) and biological efficiency(20.14%) significantly lowest Yield in multispore culture (0.900 kg/ bed) and (12.14%) biological efficiency was observed. Similar trends also observed on radial growth & biomass production culture of monosporous isolates (SSI 3)was found significantly highest radial growth (88.00 mm) and biomass production (0.420 gram) found statistically at par with tissue culture raised from JSP2 highest radial growth (84.40 mm) and biomass production (0.410 gram) isolate of V. volvacea.

Keywords: Radial growth, Biomass, Yield potential, Biological efficiency

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