CONTROL OF CONTAMINATION BY MODIFIED STERILIZATION PROCESS USING PLANT PRESERVATIVE MIXTURE IN THE IN VITRO CULTURES OF BAMBUSABALCOOA

KavyaSree Eega¹, Shalini Mudalkar¹*, Sreedhar Bodiga², Reeja Sundaram¹, Sailatha Banda¹ and Sneha Akkenapally¹

¹Department of Forest Biology and Tree Improvement, FCRI, Mulugu, Hyderabad, Telangana, India

²Department of Basic and Social sciences, FCRI, Mulugu, Hyderabad, Telangana, India

Email: Shalini.mudalkar@gmail.com

Received-01.10.2022, Revised-12.10.2022, Accepted-24.10.2022

Abstract: Bambusa balcooa was one of the commercially important sustainable bamboo species. Establishment of contamination free cultures is crucial step for the successful micro propagation procedures. The nodal explants of B. balcooa were treated with different chemicals and antibiotics. An efficient surface sterilization procedure includes the sequential use of different chemicals like 0.5% NaOCl (Sodium hypochlorite), 0.1 % Mercuric chloride treatment, 0.2% Carbendazim, 70% Ethanol resulting in healthy growth of the plants by eliminating the exogenous contamination. Among the different antibiotics incorporated into the MS media (Murashige and Skoog ,1962), addition of PPM (Plant Preservative Mixture) (0.5 ml/L) was found to be effective in controlling the contaminants like bacterial and fungal growth which reduced to 8.33±1.67%.

Keywords: Antibiotics, Contamination, Micropropagation, Plant Preservative Mixture, Surface sterilization

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