Rakesh Patel1*, N.A. Khan2, Parul Devi3, Sabia Ahad4 and D.K. Dwivedi5
Department of PMB & GE, A.N.D. University of Agriculture and Technology, Kumarganj,
Ayodhya (U.P.)-224229
Email: rakeshpatel478.rp@gmail.com
Received-03.07.2022, Revised-17.07.2022, Accepted-29.07.2022
Abstract: The primary objective of the current research investigation is to estimate the total protein content and protein profiling from non-infected and infected leaves of 10 varieties of Indian mustard (Brassica juncea L.) to check the effect due to Alternaria brassicae. The protein content was estimated by UV-Vis spectrophotometric technique using the conventional Lowry’s method. The highest protein was seen in non-infected leaves while the lowest was found in the infected leaf of moderately resistant variety (Rohini). The true protein in all varieties was found to be altered due to infection i.e. decreased. Diversity within non-infected and infected leaves of Brassica juncea can be analyzed at molecular level with the help of leaves protein by using SDS-PAGE.Total 6 bands were obtained both from samples of non-infected and infected leaves. Through statistical analyses dendrogram was formed and varieties were clustered into different groups by applying UPGMA (un-weighed pair group mean analyses).
Keywords: Indian mustard, Alternaria blight, Folin-lowery, SDS-PAGE, Protein profiling
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