Shiva Kumar R.1*,Balaji B.2 and Dharani E.3
1Department of Molecular Biology and Genetic engineering, College of Basic Sciences and Humanities, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand,
India – 263145
2Division ofMolecular Biology &Biotechnology, Indian Agricultural Research Institute,Pusa, New Delhi, India – 110012
3 Division of Plant Physiology, Indian Agricultural Research Institute – National Institute of Abiotic Stress Management, Baramati, Maharashtra, India – 413115
Email: shivakumar.r.writer@gmail.com
Received-01.05.2022, Revised-16.05.2022, Accepted-26.05.2022
Abstract: The Himalayan medicinal plant Podophyllum hexandrum is one of the most important medicinal plants under threat. Due to the presence of podophyllotoxin in its rhizome, it has its importance in the treatment of some dermatological infections and anti-cancer properties. Its rapid population decline and increasing global demand have forced alternative in vitro proliferation methods, such as somatic embryogenesis, in which different tissues are cultured using different PGRs such as auxin and cytokinin. Although these two hormones cannot act individually to initiate callus formation, auxins have been shown to trigger callus initiation in the early stages. Similarly, when auxin levels are very low, cytokinin affect the onset of callus. Therefore, for industrial production of podophyllotoxin, it is important to maintain the correct levels of auxin and cytokinin levels in the basal medium for large-scale growth. The optimal concentration range for callus culture, organ formation and somatic embryo formation is determined by the maximum growth observed in different experiments under different conditions.
Keywords: Micropropagation, Plant growth hormones, Podophyllum, Tissue culture, Somatic embryogenesis
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