Manoj Kundu, Suresh Kumar*, Rajesh Lathar
Department of Horticulture,
CCS Haryana Agricultural University, Hisar-125004 (Haryana), India
Received-07.02.2022, Revised-20.02.2022, Accepted-27.02.2022
Abstract: Aqueous solution of mercuric chloride (0.1%) was used to study its effect on sterilization of different explants of three cultivars of lilium longiflorum i.e. Elite, Brunello and Cordelia. From each cultivar three types of explants i.e. shoot tips, scales and nodal segment were treated. The treatment duration varied from one to ten minutes in all three types of explants from each cultivar. It was observed that the survival rate of scales was maximum in all the three cultivars and minimum in nodal segments. The best survival was observed when seven minutes’ treatment was given to all the cultivars used. The survival rate started to decline when time duration was more than seven minutes. For treatment duration of one minute and two minutes the survival rate was zero in all three explants used of cultivar Elite and Cordelia. In case of cultivar Brunello there was no survival in any explants for one-minute treatment duration.
Keywords: Lilium Longiflorum, Mercuric Chloride, Shoot Tips, Scales, Nodal Segment
REFERENCES
Dilta, B.S., Sehgal, O.P., Pathania, N.S. and Chander, S. (2000). In vitro effect of NAA and BA on culture establishment and bulblet formation in Lily. J. Ornam. Hort. New Series 3(2): 65-70.
Masterova, I., Vhrin, D. and Tomako, I. (1987). Phytochemistry. 26: 1844-1845.
Omamor, I.B., Asemota, A.O., Eke, C.R. and Ezia, El (2007). Fungal contaminants of the oil palm tissue culture in Nigerian institute for Oil Palm Research (NIFOR) Afr. J. Agric. Res. 2(10): 534-537.
Priyadarshi, S. and Sen, S. (1992). A revised scheme for mass propagation of Easter lily. Plant Cell, Tissue and Organ Culture 30: 1930-197.