2020, Issue 3, Volume 12

PATHOGENICITY TEST BY USING ARTIFICIAL INOCULATION METHODS AND ECO-FRIENDLY MANAGEMENT OF ALBUGO CANDIDA ON BRASSICA JUNCEA UNDER PUNJAB REGION

Amanpreet Singh, Gagandeep Singh Sohi and Bahaderjeet Singh*

Department of Plant Pathology, College of Agriculture, Guru Kashi University, Talwandi Sabo-151302, Bathinda (Punjab), India.

Email: sidhubahaderjit@yahoo.in

Received-06.03.2020, Revised-27.03.2020

Abstract: The oilseed crops especially rapeseed-mustard play a vital role in agricultural economy of the world. Diseases of plant pathogens are considered an important biotic constraint, which leads to significant yield losses of crop world-wide. Of all the agricultural pests and diseases that threaten mustard crop, white rust is one of the most devastating. White rust of mustard is caused by an obligate biotrophic fungus Albugo candida. Under technique of detached leaf inoculation, three method were used viz., spore suspension solution spray method, directly spores picking method and inoculation with infected leaf method under laboratory conditions. Directly spores picking method has found to be finest and swift method. The conventional technique like spraying method has the disadvantage of causing considerable variation in spores distribution. Trichoderma viride was found most effective in Pre Treatment than Treatment After Disease Infection while neem oil was less effective in Pre Treatment.

Keywords: Albugo candida, Brassica juncea, Eco-friendly management, Pathogenicity, Trichoderma viride

REFERENCES

Anonymous (2018). https://www.nfsm.gov.in/Ready Reckoner/Oilseeds/Stat_OS 2018.pdf.

Bal, R.S. and Kumar, A. (2014). Studies on the epidemiology of white rust and Alternaria leaf blight and their effect on the yield of Indian mustard. African Journal of Agricultural Research, 9(2):302-306.

Bhatti, D.S. and Kaur, S. (2019-20). Package of practices for Rabi crops of Punjab. PAU, Ludhiana, pp 20.

Gopi, R., Avasthe, R.K., Kapoor, C. and Kalita, H. (2016). Organic management of white rust of mustard in Sikkim Himalaya. Indian Journal of Plant Protection, 44: 116-121.

Holub, E.B., Brose, E., Tor, M., Clay, C., Crute, I.R. and Beynon, J.L. (1995). Phenotypic and genotypic variation in the interaction between Arabidopsis thaliana and Albugo candida. Molecular Plant–Microbe Interactions, 8:916–928.

Kaur, P., Jost, R., Sivasithamparam, K. and Barbetti, M.J. (2011). Proteome analysis of the Albugo candida–Brassica juncea pathosystem reveals that the timing of the expression of defence related genes is a crucial determinant of pathogenesis. Journal of Experimental Botany, 62: 1285–1298.

Kotle, S.J. (1985). Diseases of Annual Edible Oilseed Crop. Rapeseed mustard and seasame diseases. Vol.2. C.R.C. Press, Boca Raton, Florida, U.S.A., 320p.

Kumar, V., Kaushik, C.D., Saharan, G.S. and Gupta, P.P. (1995). Role of various factors in development of white rust disease of rapeseed mustard. Indian Journal of Mycology and Plant Pathology, 25: 145-148.

Pandey, P., Tewari, A.K. and Awasthi, R.P. (2013). Morphological and pathogenic variability of Albugo candida isolates causing white rust in rapeseed mustard. Diseases of mustard in mid-eastern India. International Journal of Life Sciences, 8(3): 835-838.

Raghav, C.S. (2003). Medicinal plant on touchstone. Science Reporter, Feb, 2003, pp.53-60.

Shakwar, R.C., Dinesh, S., Pathak, M. and Singh, A.K. (2012). Integrated approaches for management of foliar diseases in Indian mustard. Journal of Plant Disease Science, 7: 154-157.

Singh, D.V. and Singh, J. (1983). A technique for inoculating A. candida on lahi. Indian Phytopathology. 36: 139-140

Tuite, J. (1969). Plant Pathological Methods – Fungi and Bacteria. Burgess Publishing Company, Minneapolis, Minnesota, pp.239.