Sachin Kumar Jain1*, Kamal Khilari1, Ramji Singh1, Prashant Mishra1, Gaje Singh2,
M. K. Yadav3
1Department of Plant Pathology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Modipuram, Meerut -250 110
2Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Modipuram, Meerut -250 110
3Department of Ag. Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Modipuram, Meerut -250 110
Email: sachinjain1115@gmail.com
Received-09.07.2016, Revised-25.07.2016
Abstract: Rice root-knot nematode (Meloidogyne graminicola) is an emerging problem to rice cultivation in various rice growing areas. Rice root knot nematode has reported to cause upto 50% loss in grain yield and in severe cases it may go upto 64 per cent. An experiment was conducted to study the efficacy of bio-agents against Meloidogyne graminicola on rice variety PB-1121. It was observed that, all Trichoderma isolates at 20 and 30 gm/kg soil significantly increased the shoot length of rice plants. Trichoderma isolates were reduced the infestation of root knot nematode in rice. At 40 days after seed sowing, minimum 1.61 galls/ plant were recorded in Trichoderma isolate-V @ 30 gm/kg soil. In case of Trichoderma isolate-III @ 30 gm/kg soil, average 3.00 galls/ plant were recorded as compare to carbofuran @ 3gm/kg (4.61) and control (25.17). In vitro, at 72 hours after inoculation, maximum 96.68% larval mortality was recorded in Pseudomonas fluorescens suspension. In Trichoderma isolate-IV, 74.18% larval mortality was recorded as compare to carbofuran (95.00%).
Keywords: Rice crop, Rice root knot nematode, Meloidogyne graminicola, Management
REFERENCES
Altmare, C., Norvell, W. A., Bjorkman, T. and Harman, G. E. (1999). Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295–22. Appl. Environ. Microbe. 65:2926–2928.
Dababat, A. and Sikora, R. A. (2007). Use of Trichoderma harzianum and Trichoderma viride for the biological control of Meloidogyne incognita on tomato. Jordan Journal of Agricultural Sciences, 3 (3): 297-309.
Dabur, K. R. & Jain, R. K. (2005). Rice root nematode Meloidogyne graminicola a threat to rice-wheat cropping system. Indian Journal of Nematology 35(1):81-82.
Gaur, H. S. (2003). Root knot disease of rice and wheat: problem and management. Division of Nematology, Indian Agriculture Research Institute, New Delhi, India.
Holland, J. S., Williams, L. and Khan, A. (1999). Infection of Meloidogyne javanica by Paecilomyces lilacinus. Nematology, 22: 621-634.
Jairajpuri M. S. and Baqri, Q. H. (1991). Nemalode pests of rice, Oxford and IBH Publisher, New Delhi, India, p. 66.
Meyer, S., Robin, N., Xing, N., Richard, A., Jean, J. and James, K. (2004). Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile mortality. Nematology, 6 (1): 23-32.
Pandy, G., Pandy, R. K. and Pant, H. (2003). Efficacy of different levels of Trichoderma viride against root-knot nematode in chickpea (Cicer arientinum L). Annual Plant Protection Science, 11 (1): 96-98.
Rao, M., Reddy, P. and Nagesh, M. (1998). Evaluation of plant based formulation of Trichderma harzianum for the management of Meloidogyne incognita on egg. PlantNematology Mediterranea, 26: 59-62.
Rao, Y. S. & Biswas, H. (1973). Evaluation of yield losses in rice due to the root-knot nematode, Meloidogyne incognita. Indian Journal of Nematology 3: 74.
Saikia, Debanand Das, M. K. Sarmah, D. K. (1999). Comparative efficacy of botanicals and antagonistic fungi for the management of rice root-knot nematode, Meloidogyne graminicola. International Journal of Tropical Agriculture; 17(1/4):287-290.
Sharon, M., Bar-Eyal, M., Chet, I., Herreras-Etrella, A., Klefed, O. and Spiegel, Y. (2001). Biological control of the root-knot nematode Meloidogyne javanica by Trichderma harzianum. Phytopathology, 91(7): 687-693.
Soriano, R. S., Prot, J. C. and Matias, D. M. (2000). Expression of tolerance for Meloidogyne graminicola in rice cultivars as affected by soil type and flooding. Journal of Nematology (32): 309–317.
Vyas, R. V. and Patel, D. J. (2001). Management of root-knot nematode in banana. Indian J. Nematol. 31(1): 86-87.