J. Sailaja Rani,* M. Reddi Kumar, N.P. Eswara Reddy, T. Uma Maheswari, Y. Kavya
and C. Ramesh
Deptt. of Plant Pathology, Agricultural College, Mahanandi
Acharya N.G.Ranga Agrl. University (A.P.)
Email: pathsailaja@gmail.com
Received-12.06.2015, Revised-20.06.2015
Abstract: Plant disease management with bio agents is a non chemical and environmental safe method in agriculture. Tomatodamping-off caused by Pythium aphanidermatum (Edson) Fitz. is one of the most dreadful diseases. Six isolates of P. aphanidermatum were collected from tomato nurseries of different geographical areas in Andhra Pradesh and designated as CTR1, CTR2, KDP1, KDP2, KNL1 and KNL2. Native Trichoderma harzianum and Pseudomonas fluorescens were isolated from tomato rhizosphere using selective media. These two native bioagents were identified upto species level based on morphological characters. Among the two native antagonists, T. harzianum recorded maximum per cent inhibition on all isolates of P. aphanidermatum. Maximum inhibition was observed in CTR2 when T. harzianum was used while P. fluorescens recorded maximum inhibition on KDP2 in vitro. In vivo studies revealed that seed treatment with combination of T. harzianum and P. fluorescens was found to be effective in controlling pre and post-emergence damping-off.
Keywords: T. harzianum; P. fluorescens; Tomato Damping – off; P. aphanidermatum
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