Subek Singh, Prafull Kumar1 Ankita Trivedi and Avadhesh Kumar Koshal*2
Department of Genetics and Plant breeding, C.S. Azad University of Agriculture and Technology, Kanpur-208 002, (Uttar Pradesh) India
1Sardar Vallabhbhai Patel University of Agriculture & Technology,
Modipuram, Meerut, (Uttar Pradesh) India
2ICAR- Project Directorate for Farming Systems Research,
Modipuram, Meerut, (Uttar Pradesh) India
Received-05.04.2015, Revised-22.04.2015
Abstract: Mungbean (Vigna radiate (L.) Wilczek) is an important pulse crop in India with advancement and development of hundreds of varieties and introduction of intellectual property rights it is necessary to identifying them individually for identification and registration purposes. The present investigation was carried out during 2012-2013 in biotechnology lab, Department of genetics & Plant Breeding, C.S. Azad university of agriculture and technology, Kanpur with 13 genotypes of Mungbean K851, SML-683, KM 2272, PDM 51, T- 44, KM-2260, HUM-12, HUM-6, Pusa Vishal, IPM 02-3, IPM 2-14, KM-2241, PDM-139, for protein profiling through SDS-PAGE. In present investigation, 13 variety of Mungbean were studied for varietal identification through electrophoresis. Protein was extracted from dry seed of mungbean varieties and analysed by SDS-PAGE. On the basis of photographs, electrophoregrams, Rm values and dendograms (UPGMA cluster analysis) of banding patterns through SDS-PAGE, results found that the number of protein bands found in 13 genotypes ranged from 10 to 17 with Rm value 0.08 to 0.97 for tris soluble proteins. Protein banding pattern of tris soluble proteins was found more distinct in SDS-PAGE. In UPGMA cluster analysis all the genotypes fall in seven cluster groups. SDS-PAGE for tris soluble proteins found suitable for testing distinctness, uniformity, stability of varieties for registration and identification. On the basis of results, this can be said for characterization and identification of genotypes of mungbean, that electrophoretic profile for tris soluble proteins through SDS-PAGE was resulted distinct banding pattern and act as ‘genotypic finger printing’. Therefore, electrophoregram of tris soluble protein in SDS-PAGE was found much better for identification of genotypes in mungbean.
Keywords: Mungbean, SDS-PAGE, Varietal identification, UPGMA
REFERENCES
Afzal, M., Kawase, M., Nakayama, H. and Okuno, K. (1994). Variation in electrophoregrams of total seed protein and wx protein in foxtail millet. Breed Sci., 44: 642
Amjad, Hameed, Tariq Mohamed Shah, Baban Manzoor Atta, Nayyar Iqbal, M., Ahsanul Haq and Hina Ali (2009). Comparative seed storage protein profiling of kabuli chickpea genotypes. Pakistan J.Bot., 41 (2): 703-710.
Chauhan, P., Ram, C., Mann, A. and Sangwan, V.P. (2002). Molecular weight analysis of seed proteins of forage sorghum (sorghum bicolor (L.) Moench). Seed Science and technology, 30 (1): 11-16.
Drawn S. Luthe (2007). Electrophoretic analysis of seed proteins in the dicotyledoneae. Plant Molecular Biology Reporter, 10 (3): 254-262.
Gupta, S.K., Bala, A., Adarsh Bala and Satija, D.R. (2002). Genetic diversity in relation to protein and protein fractions in chickpea (Cicer arietinum), 29 (2): 122-135
Horax, R., Hettiarachchy, N.S., Chen, P. and Jalauddin, M. (2006). Preparation and characterization of protein isolate from compeer (Vigna unguiculeta L. Walp). Journal of Food Science, 69 (2): 114-118.
Iqbal, S.M., Ghaffor, A. and Ayub, N. (2005). Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak. J. Bot., 37: 87-96.
Omid Sofalian and Mostafa Valizeh (2009). Investigation of seed storage proteins in some wild wheat progenitors using SDS-PAGE and acid PAGE. Not. Hort. Agrobot. Cluj., 37(1) : 179-182
Prasad, R., Prasad, D. and Taneja, A.D. (2001). SDS-PAGE of soluble seed proteins for identification of cotton cultivars. Journal of the Indian Society for Cotton Improvement, 126: 2
Ritu Mahajan, Sarla, P. Malhotra and Randhir Singh (1987). Charaterization of seed proteins of urdbean (Vigna mungo). Springerlink Journal Article, 10: 163-173.
Rico Juan, Julio Pster, Manual Alaiz and Javier Vioque (2007). Electrophoretic characterization of Ameranthus L. Seed proteins and its systematic implications. Botanical Journal of the Linnean Society, 155 (1): 57-63.
Shathe, S.K. and Salumkhe, D.K. (2006). Solubilization and electrophoretic characterization of the great northern bean (Phaseolas vulgaris L.) proteins. Journal of food science, 46: 82.