Preeti, 1 Sudhir Kumar2 and J.D.S. Panwar3
| Short Communication |
1,2 Deptt. of Botany, J.V. College, Baraut, Baghpat.
3Division of Plant Physiology. IARI, New Delhi-110012
Abstract : Two varieties of urd bean (PU-35, T-9) inoculated with Rhizobium and vesicular arbuscular mycorrhiza fungi (applied through layering technique) were raised under field conditions. The synergistic effect was noticed with the combined treatment over any of the bacteria or AM fungi, in terms of chlorophyll content, photosynthetic rate and root phosphatase activity. The interaction enhanced the dry matter production, grain yield and quality also. The carbohydrate, fat and protein content also increased in the Rhizobium inoculated seeds; however, it was higher when Rhizobium and mycorrhiza fungi were combined together. Rhizobium inoculation enhanced the nitrogen content in grain and straw where as Rhizobium + AM treated plants had enhanced phosphatase activity and nitrogen content.
Keywords : AM fungi, Photosynthetic rate, Rhizobium, Root Phosphatase activity and grain quality, Urd bean
REFERENCES
Amaya-Carpio, L., Daries, F.T., Jr., Fox, T. and He, C. (2009). Arbuscular mycorrhizal fungi and organic fertilizer influence photosynthesis, root phosphatase activity, nutrition and growth of Ipomoea carnea ssp. fistulosa. Photosynthe 47(1):1-10.
Cooper, K.M. (1984). Physiology of VA-Mycorrhizal Association pp 155-203. [In] VA mycorrhiza. Ed. C.L. Powell, D.J., Bagyaraj; pp. 113-130. Boca Roton, Fla: CRC Press,London.
Giller, K.E. (2001). Nitrogen fixation in Tropical Cropping System. 2nd edition, CABI publishing. pp. 423.
Ghosh, G. and Poi, S.C.(1998). Response of Rhizobium ,PSB and mycorrhizal organisms on some legume crops. Environ Ecol.16(3):607-610.
Hiscox, J.D. and Israelstam, G.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57 (7-12); 1332-1334.
Hodge, A. (2000). Microbial ecology of arbuscular mycorrhiza. FEMS. Microbiology Ecology. 32:91-96.
Hodge, J.E. and Hofreiter, B.T. (1962). In : Method in Carbohydrate Chemistry (Ed. Whister, RL. and Bemiller, J.N.) Academic Press, New York.
Jain, A.K., Kumar, Sudhir and Panwar, J.D.S. (2007). Response of mung bean (Vigna radiata) to phosphorus and micronutrients on N and P uptake and seed quality. Legume Res.30(3):201-204.
Jain, A.K., Sudhir Kumar, O.P. Singh, J.D.S. Panwar and Vinod Kumar (2008). Studies in relation to Rhizobium and vesicular arbuscular mycorrhizal (VAM) inoculation with phosphorus in a Green Gram (Vigna radiata). Plant Archives. Vol. 8. No.2, pp 935-936.
Jain, A.K., Sudhir Kumar and J.D.S. Panwar. ( 2008). Role of vesicular arbuscular mycorrhiza (VAM) and Rock phosphate in pigeon pea. Ad. Plant Sci.21(11):411-412.
Jackson, M.L. (1958). Soil Chemical Analysis, Prantice Hall, Englewood Cliffs, N.J., USA.
Kartha, A.R.S. and Sethi, A.S. (1957). Cold percolation method for oil estimation in seeds. Indian J. Agril. Sci, 27:211.
Marschner, P., Solaiman, Z. and Rengel, Z. (2006). Rhizosphere properties of Poaceae genotypes under P-limiting condition. Plant Soil.107: 11-18.
Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153:375-380
Panday, R. (2006). Root-exuded acid phosphatase and 32Pi-uptake kinetics of wheat, rye and triticale under phosphorus starvation. Journal Nuclear Agric. Biol. 35 (3-4): 168-179.
Pandey, R., Singh, B. and Nair, T.V.R. (2006). Effect of arbuscular mycorrhizal inoculation in low phosphorus soil in relation to P urtilization efficiency of wheat genotypes. India J. Agric. Sci. 76(6): 349-353.
Panse, V.G. and P.V. Sukhatme. (1967). In “Statistical methods for agricultural workers”, Ed. Jaiswal, P.L., ICAR, New Delhi.
Panwar. J.D.S. (1993). Response of VAM and Azospirillum inoculation to water status and grain yield in wheat under water stress condition. Indian J. Plant Physiol., 36 (1):41-43.
Panwar, J.D.S. and Thakur, A.K. (1995). Physiological and biochemical studies in vesicular arbuscular mycorrhizae-Rhizobium inoculated mungbean plants under field condition. Proc Mycorrhizae biofertilizers for the future.Tata Energy Res. Institute. pp 472-477.
Radersma, S. and Grierson, P.F. (2004). Phosphorus mobilization in Agro forestry: Organic anions, phosphatase activity and phosphorus fractions in the rhizosphere. Plant Soil. 259:209-219.
Shiva Kumar, B.G. and Shiva Dhar. (2008). Microbial Transformation of nitrogen in Rhizosphere and its impact on soil fertility [In]. Rhizosphere: strategies for augumenting soil fertility and productivity. Division of Agronomy, IARI (Eds.) Shiva Dhar; Majaiah, K.M., Annapurna, K. and R.K. Rai. pp. 56-63.
Sinha, S. K., Bhargava, S. C. and Baldev, B. (1988). Physiological aspects of pulse crops. [In]. Pulse Crops. Eds. Baldev, B., Ramanujam, S. P. and Jain, H. K. Pub. Oxford and IBH, New Delhi.
Srivastava, T.K., Ahalawat, I.P.S. and Panwar, J.D.S. (1998). Effect of phosphorus, molybdenum and biofertilizers on productivity of Pea (Pisum sativum L). Ind.J.Pl.Physiol. 3(3):237-239.July-Sep.1998.
Tabatabai, M. A.(1994). Soil Enzymes.[In]: Methods in soil Analysis.Part 2(ed).Weaver,R. V.,Angle, J. S., Botomley, P.S. pp.775-833.Madison.
Thakur, A.K. (1994). Physiological studies on Rhizobium– VAM symbionts in mung bean. M.Sc. Thesis P.G. School, IARI, New Delhi.
Thakur, A.K. and Panwar, J.D.S. (1997). Response of Rhizobium – vesicular arbuscular mycorrhizal symbionts on photosynthesis, nitrogen metabolism and sucrose translocation in green gram (Phaseolus radiatus).Indian J.Agric Science.67(6):245-248.June, 1997.