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Sah Fatoumata Diomandé1, Kouakou Romaric Kouakou2, Yaya Touré3*, Pyminnan Traoré1, Tchoa Koné1 and Mongomaké Koné1
¹Laboratory of Biology and Improvement of Plant Production, Faculty of Natural Sciences, Nangui ABROGOUA University, Abidjan, Côte d’Ivoire.
2National Center for Agronomic Research (CNRA), Korhogo Regional Directorate, Ferkessédougou Research Station, Côte d’Ivoire
3Agronomy and Forestry Department, Faculty of Agricultural, Forestry and Environmental Engineering, Université of Man, Man, Côte d’Ivoire.
Received-26.07.2026, Revised-15.08.2026, Accepted-28.08.2026
Abstract: The limited availability of high-quality planting material is one of the major constraints to increasing plantain production in Côte d’Ivoire. Macropropagation represents an accessible alternative for the rapid production of planting material; however, the biochemical and enzymatic processes associated with bud formation remain poorly documented. This study evaluated the effect of prior sucker dehydration on bud production and characterized changes in selected biochemical compounds and enzymatic activities during macropropagation of the plantain (Musa AAB) cv. ‘Corne 1’. A total of 90 suckers were collected, and healthy suckers were assigned to two treatments: non-dehydrated suckers (TEM) and suckers dehydrated for two weeks (DESH). Total sugar, total protein, and total phenolic compound contents, as well as phenylalanine ammonia-lyase (PAL) and peroxidase (POX) activities, were determined weekly over a six-week incubation period. The first roots emerged after 7–8 days, whereas the first buds were observed after 14 days. The mean number of buds per explant was significantly higher in DESH (58.28 buds) than in TEM (34.66 buds). In contrast, the mean times to root emergence and bud emergence did not differ significantly between treatments. Total sugar, protein, and phenolic compound contents, as well as PAL and POX activities, showed temporal variations that differed between treatments. These results indicate that prior dehydration for two weeks can enhance bud production and is associated with changes in the biochemical and enzymatic dynamics of suckers during plantain macropropagation.
Keywords: Plantain, Musa AAB, Macropropagation, Sucker dehydration, Bud production, Biochemical changes
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