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Abeeda Mushtaq, Vikas Sharma*, Chandan Thappa, Proloy Sankar Dev Roy and Komal Sudan
Natural Products Laboratory, Division of Biochemistry, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus,
Chatha, Jammu – 180009, J&K
Email: vikas.skuast@gmail.com
Received-04.09.2025, Revised-16.09.2025, Accepted-29.09.2025
Abstract: The present study was carried out to evaluate the in vitro antibacterial potential of rhizome and shoot part of Acorus calamus via five different extracts namely n-hexane, chloroform, ethyl acetate, acetone and methanol. The agar-well diffusion method was used against two-gram positive (Bacillus cereus MTCC5981, Staphylococcus aureus MTCC96) and three-gram negative (Escherischia coli MTCC1697, Klebsiella pneumoniae MTCC109, Pseudomonas aeruginosa MTCC741) bacterial strains and the findings from broth dilution assay for different plant extracts demonstrated antibacterial properties on the tested strains of bacteria at various degrees, as displayed by their minimum inhibitory concentration (MIC) values. The MIC values for rhizome part of A. calamus were in range of 410-835 μg/ml for all the tested bacterial strains, but the significant value was displayed by methanolic extract. For A. calamus shoot part, MIC values against the five tested bacterial strains ranged from 415-880 μg/ml, but the acetone extract was found to be the most effective against all bacterial strains as it exhibited the lowest MIC values. The results of present study revealed that rhizome and shoot part of Acorus calamus possess antibacterial potential and source of new antibiotics. Therefore, medicinal plants are finding their way into pharmaceuticals, nutraceuticals and food supplements that could be useful in chemotherapy to control infectious diseases.
Keywords: Acorus calamus, Antibiotics, Bacterial strains, Minimum inhibitory concentration
REFERENCES
Chandra, D. and Prasad, K. (2017). Phytochemicals of Acorus calamus (Sweet flag). Journal of Medicinal Plants Studies, 5(5), 277-281.
Eloff, J. N. (2019). Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results. BMC Complementary and Alternative Medicine, 19: 1-8.
Feyza, O., Belma, A., Sahlan, O. and Senol, A. (2009). Essential oil composition of antimicrobial and antioxidant activity of Satureja cunei folia. Food Chemistry, 112:874-879.
Imam, H., Riaz, Z., Azhar, M., Sofi, G. and Hussain, A. (2013). Sweet flag (Acorus calamus Linn.): An incredible medicinal herb. International Journal of Green Pharmacy,7(4): 288-296.
Rajput, S. B., Tonge, M. B. and Karuppayil, S. M. (2014). An overview on traditional uses and pharmacological profile of Acorus calamus Linn. (Sweet flag) and other Acorus species. Phytomedicine, 21(3): 268-276.
Singh, R., Sharma, P. K. and Malviya, R. (2011). Pharmacological properties and ayurvedic value of Indian buch plant (Acorus calamus): a short review. Advances in Biological Research, 5(3): 145-154.
Amit Tomar (2007). Some medicinal plants used as an Antipyretic among the rural and common people in Meerut District of Western Uttar Pradesh. Journal of Non-Timber Forest Product, 14(3): 215-218.
DOI: https://doi.org/10.54207/bsmps2000-2007-C7247N
Amit Tomar (2008). Folk medicinal plants in Muzaffarnagar district of Western Uttar Pradesh, India. Journal of the Indian Botanical Society, 87 (3 & 4): 200-208.
Tomar, A. (2017). Medicinal use of Acorus calamus L. (Bach) to cure fever. Journal of Non-Timber Forest Products, 24(1):47-48.
DOI: https://doi.org/10.54207/bsmps2000-2017-U9BF76
Ugboko, H. U., Nwinyi, O. C., Oranusi, S. U., Fatoki, T. H. and Omonhinmin, C. A. (2020). Antimicrobial importance of medicinal plants in Nigeria. The Scientific World Journal, 2020: 7059323.
Umamaheshwari, N. and Rekha, A. (2018). Sweet flag: (Acorus calamus)-An incredible medicinal herb. Journal of Pharmacognosy and Phytochemistry, 7: 15-22.
Vaou, N., Stavropoulou, E., Voidarou, C., Tsigalou, C. and Bezirtzoglou, E. (2021). Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms, 9: 2041-2068.