2025, Issue 8, Volume 17

PLANT GROWTH AND DEVELOPMENT IN SPACE: CHALLENGES AND OPPORTUNITIES

View: Full Length Article

Bratislav Stankovic1*

1Bratislav Stankovic, United States Patent and Trademark Office, Alexandria, VA 22314, USA

* Bratislav Stankovic contributed to this article in his personal capacity; the views expressed are his own and do not necessarily represent the views of the USPTO or the US Government.

Email: bratislav.stankovic@fulbrightmail.org

Received-28.07.2025, Revised-15.08.2025, Accepted-29.08.2025

Abstract: The growth of plants in outer space has elicited much scientific interest. Plants are expected to be essential for sustaining human life during extended space missions, likely starting soon with a journey to Mars. Given their ability to endure harsh conditions on Earth and evidence that model plants can complete their life cycle in microgravity, plants are well-suited for space-based life support systems. Their role goes beyond biology—successfully cultivating plants in space also reflects our technological ability to (re)create supportive living environments in microgravity. This knowledge also benefits humanity on Earth: by understanding how to grow plants in harsh conditions in space, we could apply that knowledge to growing agriculturally important crops in challenging conditions on Earth. Decades of research have revealed many challenges of growing plants in microgravity, space and on other celestial bodies, but our understanding of this endeavor has improved significantly. As space exploration moves forward, we can be increasingly confident in our capability to cultivate plants aboard spacecraft, on the Moon and beyond. Although plants will need specially designed environments to thrive in microgravity, we already possess the knowledge and tools to create such systems on a small scale.

Keywords: Space biology, Gravitational biology, Microgravity, Plant development, Spaceflight

REFERENCES

Bates, S., Gushin, V., Bingham, G, Vinokhodova, A, Marquit, J. and Sychev, V. A. (2009) Review of the literature and application to habitation systems for humans living in isolated or extreme environments. Habitation, 12: 33-40.

Google Scholar

Beischer, D.E. and Fregly, A.R. (1962) Animals and man in space. A chronology and annotated bibliography through the year 1960. US Naval School of Aviation Medicine. Pensacola, FL. ONR REPORT ACR-64 (AD272581).

Google Scholar

Brown, C.S., Tipathy, B.C. and Stutte, G.W. (1996) Photosynthesis and carbohydrate metabolism in microgravity. In: Plants in Space Biology (eds. H. Suge and H. Takahashi). Tohoku University Press, Sendai, Japan, pp. 127-134.

Google Scholar

Brown, C.S., Winter-Sederoff, H., Davies, E. and Stankovic, B. (2008) Plan(t)s for space exploration. In: Simon Gilroy and Patrick H Masson, editors. Wiley-Blackwell Publishing; p 183-195.

Google Scholar

Buckner, A., Lang, S. and Loureiro, R. (2025) Physiological and transcriptional responses of Arabidopsis thaliana to simulated lunar and Martian regolith substrates. arXiv:2505.13583 [q-bio.QM].

Google Scholar

Campbell, W.F., Salisbury, F.B., Bugbee, B., Klassen, S., Naegle, E., Strickland, D.T., Bingham, G.E., Levinskikh, M., Iljina, G.M., Veselova, T.D., Sytchev, V.N., Podolsky, G., McManus, W.R., Bubenheim, D.L., Stieber, J. and Jahns, G. (2001) Comparative floral development of Mir-grown and ethylene-treated, earth-grown Super Dwarf wheat. Journal of Plant Physiology, 158: 1051-1060.

Google Scholar

Chebli, Y. and Geitmann, A. (2011) Gravity research on plants: use of single-cell experimental models. Frontiers in Plant Science, 2(56): 1-10.

Google Scholar

Darrin, A. and O’Leary, B.L. (2009) Handbook of Space Engineering, Archaeology and Heritage. Boca Raton: CRC Press; 1035 p.

Google Scholar

De Micco, V., Aronne, G., Colla, G., Fortezza, R. and De Pascale, S. (2009) Agro-biology for bioregenerative life support systems in long-term space missions: general constraints and the Italian efforts. Journal of Plant Interactions, 4: 241-252.

Google Scholar

Dong, C., Shao, L., Fu, Y., Wang, M., Xie, B., Yu, J. and Liu, H. (2015) Evaluation of wheat growth, morphological characteristics, biomass yield and quality in Lunar Palace-1, plant factory, green house and field systems. Acta Astronautica, 111: 102-109.

Google Scholar

Halstead, T.W. and Dutcher, F.R. (1987) Plants in space. Annual Review of Plant Physiology, 38: 317-345.

Google Scholar

Hoson, T., Soga, K., Wakabayahis, K., Kamisaka, S. and Tanimoto, E. (2003) Growth and cell wall changes in rice roots during spaceflight. Plant and Soil, 255: 19-26.

Google Scholar

Hoson, T. (2014) Plant growth and morphogenesis under different gravity conditions: relevance to plant life in space. Life, 4: 205-216.

Google Scholar

Johnson, S.P. and Tibbitts, T.W. (1968) The liminal angle of a plagiogeotropic organ under weightlessness. Bioscience, 18: 655-661.

Google Scholar

Karoliussen, I., Brinckmann, E. and Kittang, A-I. (2013) Will plants grow on Moon or Mars? Current Biotechnology, 2: 235-243.

Google Scholar

Kiss, J., Edelmann RE and Wood PC. (1999) Gravitropism of hypocotyls of wild-type and starch-deficient Arabidopsis seedlings in spaceflight studies. Planta, 209: 96-103.

Google Scholar

Klymchuk, D.O., Vorobyova, T.V., Chapman, D.K. and Brown, C.S. (2003) Changes in vacuolation in the root apex cells of soybean seedlings in microgravity. Advances in Space Research, 31: 2283-2288.

Google Scholar

Kordyum, E.L. and Chapman, D.K. (2017) Plants and microgravity: patterns of microgravity effects at the cellular and molecular levels. Cytology and Genetics, 51(2): 108-116.

Google Scholar

Krikorian, A.D. and O’Connor, S.A. (1984) Karyological observations. Annals of Botany, 54: 49-63.

Google Scholar

Kuang, A., Xiao, Y., McClure, G. and Musgrave, M.E. (2000) Influence of microgravity on ultrastructure and storage reserves in seeds of Brassica rapa L. Annals of Botany, 85: 851-859.

Google Scholar

Lang, S., Buckner, A., Jones, S., Erwin, G., Lee, S. and Loureiro, R. (2025) Inkspot: A stress-resilient, anthocyanin rich, dwarf tomato variant for off-world cultivation. arXiv:2505.16890 [q-bio.OT].

Google Scholar

Lewis, C.A. (1995) Human health and well-being: the psychological, physiological and sociological effects of plants on people. Acta Horticulturae, 391: 31-39.

Google Scholar

Link, B.M. and Cosgrove, D.J. (2000) Analysis of peg formation in cucumber seedlings grown on clinostats and in a microgravity (space) environment. Journal of Plant Research, 112: 507-516.

Google Scholar

Link, B.M., Durst, S.J., Zhou, W. and Stankovic, B. (2003) Seed-to-seed growth of Arabidopsis thaliana on the International Space Station. Advances in Space Research, 31: 2237-2243.

Google Scholar

Link, B.M., Busse, J.S. and Stankovic, B. (2014) Seed-to-seed-to-seed growth and development of Arabidopsis in microgravity. Astrobiology, 14: 866-875.

Google Scholar

Llorente, B., Williams, T.C. and Goold, H.D. (2018) The Multiplanetary Future of Plant Synthetic Biology. Genes, 10; 9(7):348.

Google Scholar

Mashinsky, A., Ivanova, I., Derendyaeva, T., Nechitailo, G. and Salisbury, F. (1994) “From seed-to-seed” experiment with wheat plants under space-flight conditions. Advances in Space Research, 14: 13-19.

Google Scholar

Merkys, A.L. and Laurinavicius, R.S. (1983) Complete cycle of individual development of Arabidopsis thaliana (L.) Heynh. Plants on board the Salyut-7 orbital Station. Doklady Akademii Nauk SSSR, 271: 509-512.

Google Scholar

Moore, R., McClelen, C.E., Fondren, W.M. and Wang, C.L. (1987) Influence of microgravity on root-cap regeneration and the structure of columella cells in Zea mays. American Journal of Botany, 74: 218-223.

Google Scholar

Morphew, M.E. (2001) Psychological and Human Factors in Long Duration Spaceflight. McGill Journal of Medicine, 6: 74-80.

Google Scholar

Morrow, R. (2014) A brief history of growing plants in space. Engineering and Technology for Sustainable World. American Society of Agricultural and Biological Engineers, 3: 17-19.

Google Scholar

Musgrave, M.E., Kuang, A. and Matthews, S.W. (1997) Plant reproduction during spaceflight: importance of the gaseous environment. Planta, 203: S177-S184.

Google Scholar

Musgrave, M.E., Kuang, A., Brown, C.S. and Mathews, S.W. (1998) Changes in Arabidopsis leaf ultrastructure, chlorophyll and carbohydrate content during spaceflight depend on ventilation. Annals of Botany, 81: 503-512.

Google Scholar

Musgrave, M. (2007) Growing plants in space. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 2(065), 9.

Google Scholar

NASA Vegetable Production System (Veggie) [Internet] (2017) Available from: https://www.nasa.gov/mission_pages/station/research/experiments/383.html  [Accessed 2017-11-15].

Google Scholar

NASA Advanced AstrocultureTM (ADVASC) Fact Sheet [Internet] (2001) Available from: http://www.nasa.gov/centers/marshall/news/background/facts/advasc.html [Accessed 2017-11-15]

Google Scholar

NASA Advanced Plant Habitat [Internet] (2017) Available from: https://www.nasa.gov/mission_pages/station/research/experiments/2302.html [Accessed 2017-11-15].

Google Scholar

Nie, H., Zhou, W., Zheng, Z., Deng, Y., Zhang, W., Zhang, M., Jiang, Z., Zheng, H., Yuan, L., Yang, J. and Wang, H. (2025) Exploring plant responses to altered gravity for advancing space agriculture. Plant Communications, 6: 101370.

Google Scholar

Odeh, R. and Guy, C. (2017) Gardening for therapeutic people-plant interactions during long-duration space missions. Open Agriculture, 2: 1-13.

Google Scholar

Paul, A-L., Zupanska A.K., Ostrow, D.T., Zhang, Y., Sun, Y., Li, J-L., Shanker, S., Farmerie, W.G., Amalfitano, C.E. and Ferl, R.J. (2012) Spaceflight transcriptomes: unique responses to a novel environment. Astrobiology, 12: 40-56.

Google Scholar

Paul, A-L., Zupanska, A.K., Schultz, E.R. and Ferl, R.J. (2013) Organ-specific remodeling of the Arabidopsis transcriptome in response to spaceflight. BMC Plant Biology, 13: 112.

Google Scholar

Perchonok, M. and Bourland, C. (2002) NASA food systems: past, present and future. Nutrition, 18: 913-920.

Google Scholar

Porterfield, D.M., Matthews, S.W., Daugherty, C.J. and Musgrave, M.E. (1997) Spaceflight exposure effects on transcription, activity and localization of alcohol dehydrogenase in the roots of Arabidopsis thaliana. Plant Physiology, 113: 685-693.

Google Scholar

Porterfield D.M., Dreschel, T.W. and Musgrave, M.E. (2000) A ground-based comparison of nutrient delivery technologies originally developed for growing plants in the spaceflight environment. Horticulture Technology, 10: 179-185.

Google Scholar

Porterfield, D.M. (2002) The biophysical limitations in physiological transport and exchange in plants grown in microgravity. Journal of Plant Growth Regulation, 21: 177-190.

Google Scholar

Poulet, L., Fontaine, J.-P. and Dussap, C.-G. (2016) Plant’s response to space environment: a comprehensive review including mechanistic modelling for future space gardeners. Botany Letters, 163: 337-347.

Google Scholar

Rea, G., Cristofaro, F., Pani, G., Pascucci, B., Ghuge, S.A., Corsetto, P.A., Imbriani, M., Visai, L. and Rizzo, A. (2016) Microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment. Journal of Proteomics, 137: 3-18.

Google Scholar

Ruyters, G. and Braun, M. (2014) Plant biology in space: recent accomplishments and recommendations for future research. Plant Biology, 16: 4–11.

Google Scholar

Sager, J.C. and Drysdale, A.E. (1996) Concepts, components and controls for a CELSS. In: Plant Production in Closed Ecosystems: the International Symposium on Plant Production in Closed Ecosystems (eds E. Goto, K. Kurata, M. Hayashi and S. Sase) Kluwer Academic, Dordrecht, The Netherlands; pp. 205-223.

Google Scholar

Shi, J., Lu, W. and Sun, Y. (2014) Comparison of space flight and heavy ion radiation induced genomic/epigenomic mutations in rice (Oryza sativa). Life Sciences in Space Research, 1: 74-79.

Google Scholar

Soga, K. and Wakabayashi, K. (2002) Stimulation of elongation growth and xyloglucan breakdown in Arabidopsis hypocotyls under microgravity conditions in space. Planta, 215: 1040-1046.

Google Scholar

Stankovic, B. (2001) 2001: A plant space odyssey. Trends in Plant Science, 6: 591-593.

Google Scholar

Stout, S.C., Porterfield, D.M., Briarty, L.G, Kuang, A. and Musgrave, M.E. (2001) Evidence of root zone hypoxia in Brassica rapa L. grown in microgravity. International Journal of Plant Science, 162: 249–255.

Google Scholar

Stutte, G., Wheeler, R., Morrow, R. and Newsham, G. (2011) Operational evaluation of VEGGIE food production system in the habitat demonstration unit. Proceedings of the 41st International Conference on Environmental Systems, Portland, Oregon; 3357.

Google Scholar

Ueda, J., Miyamoto, K., Yuda, T., Hoshino, T., Sato, K., Fujii, S., Kamigaichi, S., Izumi, R., Isioka, N., Aizawa, S., Yoshizaki, I., Shimazu, T. and Fukui, K. (2000) STS-95 space experiment for plant growth and development and auxin polar transport. Biological Sciences in Space, 14: 47-57.

Google Scholar

Ulrich, R.S., Simons, R.F., Losito, B.D., Fiorito, E., Miles, M.A. and Zelson, M. (1991) Stress recovery during exposure to natural and urban environments. Journal of Environmental Psychology, 11: 201-230.

Google Scholar

Vandenbrink, J.P. and Kiss, J. (2016) Space, the final frontier: a critical review of recent experiments performed in microgravity. Plant Science, 243: 115-119.

Google Scholar

Volkmann, D., Behrens, H.M. and Sievers, A. (1986) Development and gravity sensing of cress roots under microgravity. Naturwissenschaften, 73: 438-441.

Google Scholar

Wamelink, G.W.W., Frissel, J.Y., Krijnen, W.H.J., Verwoert, M.R. and Goedhart, P.W. (2014) Can plants grow on Mars and the Moon: a growth experiment on Mars and Moon soil simulants. PLOS ONE, 9(8): e103138.

Google Scholar

Wang, L., Zang, X. and Zhou, J. (2022) Synthetic biology: A powerful booster for future agriculture. Advanced Agrochem., 1: 7-11.

Google Scholar

Weise, S.E. and Kiss, J.Z. (1999) Gravitropism of inflorescence stems in starch-deficient mutants of Arabidopsis. International Journal of Plant Science, 160: 521-527.

Google Scholar

Wheeler, R.M. (2017) Agriculture for space: people and places paving the way. Open Agriculture, 2: 14-32.

Google Scholar

Williams, D.R. (2002) Isolation and integrated testing: An introduction to the Lunar-Mars life support test project. In: Isolation NASA experiments in closed-environment living, science and technology series, Edited by: Lane, HW, Sauer, RL and Feeback, DL., Vol. 104, 1 – 6. San Diego: Univelt Incorporated.

Google Scholar

Wolff SA, Coelho L H, Karoliussen I, Jost A-IK. (2014) Effects of the extraterrestrial environment on plants: recommendations for future space experiments for the MELiSSA higher plant compartment. Life, 4: 189-204.

Google Scholar

Wolverton, C and Kiss, J. (2009) An update on plant space biology. Gravitational Space Biology, 22: 13-20.

Google Scholar

Zabel, P., Bamsey, M., Schubert, D. and Tajmar, M. (2016) Review and analysis of over 40 years of space plant growth systems. Life Sciences in Space Research, 10: 1-16.

Google Scholar

Zhou, W., Durst, S.J., De Mars, M., Stankovic, B., Link, B.M., Tellez, G., Meyers, R.A., Sandstrom, P.W. and Abba, J.R. (2002) Performance of the Advanced ASTROCULTURE plant growth unit during ISS-6A /7A mission. SAE Technical Paper 2002-01-2280.

Google Scholar