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Vikas Sharma1*, Navneet Kour1, Komal Sudan1, Shashank K Singh2 andShivangi Sharma3
1Natural Products Laboratory, Division of Biochemistry, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha,
Jammu-180 009, J&K, India
2Cancer Pharmacology Division and Natural Product Chemistry Division,
Indian Institute of Integrative Medicine, Canal Road Jammu-180001, J&K, India
3 Department of Chemistry,Govt. Degree College, RS Pura -181102, Jammu,
Jammu & Kashmir, India
Email: vikas.skuast@gmail.com
Received-28.04.2025, Revised-14.05.2025, Accepted-27.05.2025
Abstract: In the search of potential anticancer agents from minor fruits, the present research work was carried out to examine the in vitro cytotoxic potential of Aegle marmelos (bael) against nine human cancer cell lines from eight different origins such as MCF-7 and T-47D (breast), SF-295 (CNS), HCT-116 (colon), A-549 (lung), MDA-MB-435 (melanoma), OVCAR-5 (ovary), PC-3 (prostate), A-498 (renal). Methanolic extract of bael fruit was prepared and in vitro anticancer activity was determined via SRB assay at 100 μg/ml. Cells were allowed to grow for 24 h on 96-well flat bottom tissue culture plates and cells were further allowed to grow in the presence of test material for 48 h. Cell growth was terminated by addition of 50% (w/v) tricarboxylic acid and cells were stained with SRB dye. Excess dye was removed by washing with 1% (v/v) acetic acid and bound dye was dissolved in Tris buffer. OD was taken at 540 nm.Aegle marmelos displayed in vitro cytotoxic effect against colon, lung, melanoma and renal cancer cells and at lower concentrations of 50, 30, 10 and 1 μg/ml, the fruit exhibit significant in vitro cytotoxic effect against lung cancer cells (A-549). Further, IC50 value was calculated and it was observed that the bael fruit showed least IC50 value in case of lung cancer cells. To conclude, bael, possess certain constituents with cytotoxic properties and isolation / characterization of active ingredients is required for the development of anticancer drugs.
Keywords: Aegle marmelos, Bael, Cancer Cells, SRB assay, In vitro cytotoxicity
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