Sadiya Samar, Aditi and Ashok Kumar*

Department of Botany, Chaudhary Charan Singh University, Meerut (U.P) – 250004, India

Email: dr.ashokbotany@gmail.com

Received-01.03.2025, Revised-16.03.2025, Accepted-30.03.2025

Abstract: In this study, we examine the effects of Rhizobium and Phosphate Solubilizing Bacterium (PSB) on the attributes of the soil and the growth of Cicer arietinum L. (Chickpea). In this experimental work, the Rhizobium and PSB were used individually and in two combined inoculations. We measured various morphological, physiological and biochemical parameters using standard protocols. The findings of this field experiment showed that the test crop’s biomass, plant height (42.6 %), grain yield (50 %), plant nitrogen (35.14 %), phosphorus, and carbon content (24.62 %), as well as the plant protein content (49.56 %), all had significantly increased (20-40%) by the dual inoculated plant. The soil’s nutrient content was also improved. The data obtained from this experimental work indicates that the crops inoculated with a combined application of Rhizobium and PSB had higher yield and growth than single inoculants. So their combined application can be a better substitute for chemical fertilizers.

Keywords: Rhizobium, PSB, Dual inoculation, Cicer arietinum

REFERENCES

Abbasi, M. K., Musa, N. and Manzoor, M. (2015). Mineralization of Soluble P Fertilizers and Insoluble Rock Phosphate in Response to Phosphate-Solubilizing Bacteria and Poultry Manure and Their Effect on The Growth and P Utilization Efficiency of Chilli (Capsicum annuum L.). Biogeosciences, 12 (15), 4607-4619.

Google Scholar

Abid, K., Sultan, T., Kiani, M. Z., Ahmad, S., Tabassam, T. and Hassan, M. U. (2016). Effect of Rhizobium and Phosphate Solubilizing Bacteria at Different Levels of Phosphorus Applied on Nodulation, Growth and Yield of Peas (Pisum sativum). International Journal Biosciences, 8(5), 112-121.

Google Scholar

Crusciol, C. A. C., Ferrari, J., Mui, T. S., Franzluebbers, A. J., Costa, C. H. M. D., Castro, G. S. A., Ribeiro, L. C. and Costa, N. R. (2018). Rhizobial Inoculation and Molybdenum Fertilization in Peanut Crops Grown in a No Tillage System After 20 Years of Pasture. Revista Brasileira de Ciencia do Solo, 43, e0170399.

Google Scholar

Datta, N. P., Khera, M. S. and Saini, T. R. (1962). A Rapid Colorimetric Procedure for the Determination of Organic Carbon in Soils. Journal of the Indian Society of Soil Science, 10(1), 67-74.

Google Scholar

DES, MOAF and W. (2023). Directorate of economics and statistics. Crop production statistics information system, Ministry of Agriculture & Farmers Welfare, India.

Google Scholar

Diep, C.N., Duong, B.S., Nguyen, B. T. and PhanVăn, H. L. (2016). Effects of Rhizobia and Phosphate-Solubilizing Bacteria on Soybean (Glycine max L. Merr.) Cultivated on Ferralsols of Daklak Province, Vietnam. World Journal of Pharmacy and Pharmaceutical Sciences; 5 (4), 318-333.

Google Scholar

Hageman, R. H. and Reed, A. J. (1980). Nitrate Reductase from Higher Plants. In A. S. Pietro (Ed.). Methods in Enzymology (Vol. 69, pp. 270-280). Academic Press.

Google Scholar

Heisnam, P., Sah, D., Moirangthem, A., Singh, M. C., Pandey, P. K., Mahato, N. K., Longjam, R. and Pandey, A. K. (2017). Effects of Rhizobium, PSB Inoculation and Phosphorus Management on Soil Nutrient Status and Performance of Cowpea in Acid Soil of Arunachal Pradesh, India. International Journal of Current Microbiology and Applied Sciences, 6, 937-942.

Google Scholar

International Seed Testing Association (1976). International Rules for Seed Testing. Seed Science and Technology, 4, 51-177.

Google Scholar

Jangir, C. K., Singh, D. P., Meena, R. H. and Yadav, M. (2017). Effect of Fertility Levels and Biofertilizers on Physical and Chemical Properties of Soil Under Black Gram (Vigna mungo L.).  International Journal of Current Microbiology and Applied Sciences, 6(3), 223-228.

Google Scholar

Katiyal, D., Kuar, S. and Singh, N. (2020). Effect of Rhizobium and PSB inoculation on growth, yield attributes and yield of chickpea (Cicer arietinum L.). International Journal of Chemical Studies, 8(4), 3729-3734.

Google Scholar

Kaur, J., Khanna, V., Kumari, P. and Sharma, R. (2015). Influence of Psychrotolerant Plant Growth Promoting Rhizobacteria (PGPR) As Coinoculants with Rhizobium on Growth Parameters and Yield of Lentil (Lens culinaris Medikus). African Journal of Microbiology Research, 9(4), 258–264.

Google Scholar

Nissa, S. un., Bashir, S., Dar, S. A., Baba, J. A., Hakeem, S. A., Wani, R. A., Mughal, N.A. and Basu, Y. A. (2017). Effect of Rhizobiumand PSB in Combination with Phosphorus on the Enrichment of Soil Fertility and Its Effect on Yield of Green Gram (Vigna radiata L.). International Journal of Current Microbiology and Applied Sciences, 6(11), 3648-3652.

Google Scholar

Pandey, I. B., Pandey, R. K. and Kumar, R. (2015). Integrated Nutrient Management for Enhancing Productivity and Profitability of Long Duration Pigeon-Pea (Cajanus cajan) Under Rainfed Condition. Indian Journal of Agronomy, 60(3), 436-442.

Google Scholar

Pawar, P. U., Kumbhar, C. T., Patil, V. S. and Khot, G. G. (2018). Effect Of Co-Inoculation of Brady Rhizobium japonicum and Pseudomonas fluorescens on Growth, Yield and Nutrient Uptake in Soybean [Glycine max (L.) Merrill]. Crop Research, 53(1and 2), 57-62.

Google Scholar

Rajaa, D. and Takankhar, V. G. (2017). Growth Characters of Soybean (Glycine max) As Effect by Liquid Biofertilizers (Brady Rhizobium and PSB). Indian Journal of Pure and Applied Biosciences, 5(5), 101-111.

Google Scholar

Rasool, S. and Singh, J. (2016). Effect of Bio-Fertilizers and Phosphorus on Growth and Yield of Lentil (Lens culinaris L.). International Journal of Advanced Agricultural Science and Technology, 3(7), 35-42.

Google Scholar

Samar, S. and Kumar, A. (2020). Co-inoculation Potential Impact of PSB and Rhizobium on Physico-chemical Properties of Soil and Legume Crop Growth. Research Journal of Agricultural Sciences. 11(1), 01-09.

Google Scholar

Singh, A. K., Singh, S. S., Prakash, V. E. D., Kumar, S. and Dwivedi, S. K. (2015). Pulses Production in India: Present Status, Sent Status, Bottleneck and Way Forward. Journal of Agri Search, 2(2), 75-83.

Google Scholar

Singh, N., Singh, G., Aggarwal, N. and Khanna, V. (2018). Yield Enhancement and Phosphorus Economy in Lentil (Lens culinaris Medikus) with Integrated Use of Phosphorus, Rhizobium and Plant Growth Promoting Rhizobacteria. Journal of Plant Nutrition, 41(6), 737-748.

Google Scholar

U.S.D.A. (2018). USDA Food and Nutrient Database for Dietary Studies 2017-2018. Food Surveys Research. Retrieved from www.ars.usda.gov/nea/bhnrc/fsrg.

Google Scholar

Ziyaul, N., Lalita and Kumar, A. (2017). Influence of Dual Inoculation of Rhizobium and Mycorrhiza on Physiological and Biochemical Properties of Vigna radiata (L). International Journal of Recent Scientific Research, 8(12), 22633-22639.

Google Scholar