2024, Issue 4, Volume 16

ASSESSMENT OF DIFFERENT GROWTH MEDIA ON CORYNESPORA CASSIICOLA UNDER IN VITRO TEST

Paras Sangani1, Prashant B. Sandipan*2, Pushpa Ruwali 3, R.K. Patel4, P.S. Patel 5, Twinkle D. Manavadria6, Nirva Patel7 and Chitra Sharma8

1, 6 & 7 Department of Plant Pathology, N. M. College of Agriculture, Navsari Agricultural University (NAU), Navsari 396 450 (Gujarat), India

2 & 5 Main Cotton Research Station (MCRS), Navsari Agricultural University (NAU), Surat 395 007 (Gujarat), India

3 Department of Biotechnology, M. B. Government P. G. College, Haldwani 263 139,

(Uttarakhand), India

4 Krishi Vigyan Kendra, Navsari Agricultural University (NAU), Surat 395 007 (Gujarat), India

8 International Horticulture Innovation and Training Centre, Durgapur, Jaipur – 302 018 (Rajasthan), India

Received-02.03.2024, Revised-05.04.2024, Accepted-21.04.2024

Abstract: Cotton (Gossypium hirsutum L.) is one of the world’s most important fiber crops with a significant economic and social impact. It is the world’s oldest commercial crop, which provides fiber for mankind’s garments. It is also known as “The king of fibers” or “The white gold”. Cotton is primarily a raw material for a thriving textile industry and is also one of the most ancient and essential commercial crops, next to food grains. Among which, the Corynespora leaf spot of cotton caused by Corynespora cassiicola is observed in moderate form causing considerable damage in recent times but the disease has the potential to be severe and it could become significant concern for the cotton growers. Here in this experiment, seven different growth media were tested for their suitability for the mycelial growth, colony colour, cultural characteristics and sporulation of the C. cassiicola under in vitro test. Among the seven different growth media tested, Potato dextrose agar and V-8 Juice media proved the best growth media for the mycelial growth of the pathogen. The best sporulation of C. cassiicola was observed in Potato dextrose agar media, while poor sporulation was observed in Nutrient agar media.

Keywords: Cotton, Gossypium spp, Treatment, Disease, Media, Sporulation

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